FISH

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Introduction:


FISH is a molecular cytogenetic technique that uses fluorescently labeled DNA probes to target genes or chromosomal regions of interest in order to detect copy numbers (gain, loss, amplification) and the location of the specific DNA sequence within the interphase nucleus or metaphase chromosomes. FISH can be used to detect or confirm aneuploidy, microdeletions, inversions, duplications, amplification, chromosomal rearrangements, and to refine chromosomal breakpoints.  One big advantage of FISH is that nondividing cells can be used for determining copy numbers and the presence of gene amplification.  This is a highly informative technique since many genetic disorders and cancer types have known chromosomal abnormalities that can be easily identified by this method.