Reverse Dot Blot

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Reverse dot blot is a spot hybridization technique put forward by Saiki, the technology uses a membrane cured with a variety of specific probe to hybridize with the amplification target sequence. It can make screen a variety of DNA mutations in a hybridization at the same time.

The traditional hybridization can screen one kind of mutation only. And this new technology changes the old mode. Reverse dot blot is fast, simple, of high sensitivity, and strong specificity. In the detection of gene mutation, genotyping, pathogens and other fields, reverse dot bolt has its unique advantage.

 

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