Diagnostic Kit For M.tuberculosis Rifampicin Resistance

  • Print
  • Email

The genetic basis for RMP resistance in approximately 95 percent of the cases is due to mutations in an 81-bp rifampicin resistance determining region (RRDR) of the rpoB gene corresponding to 507-533 codons that codes for the b subunit of RNA polymerase of M. tuberculosis.
The Life-Daan JV Rifampicin Resistant Mutations Detection Kit targets this 81bp RRDR region, and can detect 129 known mutations associated with resistance to Rifampicin.

Identify known Rifampicin resistance mutations


  • Low–input DNA protocol (20 ng), compatible with sputum samples
  • Technology: Sanger Sequencing
  • Test results for research use only, cannot be taken as the basis of clinical diagnosis.

【How It Works】

  • Amplification of relevant portions of the RRDR region, which corresponding to 507-533 codons of rpoB gene;
  • Gene sequencing with 3500Dx and 3500Dx XL Genetic Analyzer of purified products;
  • Computer analysis to determine mutation presence or absence.

【Applicable population】

  • Patients who use anti-TB drugs for long period
  • Patients who have non-standard treatment
  • Chronic tuberculosis patients (after multiple irregular treatment but still MTB positive)
  • Smear positive patients who have close contact with tuberculosis patients, For persons who have been exposed to INH-resistant, RIF-susceptible TB and are known or suspected to have latent TB infection
  • Smear positive patients for retreatment (including those went through a retreatment failure).
  • Smear positive patients (first-time treatment) who still have sputum smear positive after 3 months treatment.