Single point mutations and chromosome translocations, as well as mutant and wild type
Yes. Our specific designed Taqman probe can screen for the single point mutation, gene frame-shift mutation, deletion mutation, gene translocation, heterozygous alleles, and wild type on the same allele sequence.
Specific PCR products and by-products must be able to be differentiated by the device by means of melting curve analysis
Yes. It has melting curve analysis which can be used to discriminate target PCR product and by-product.
The characteristics of qPCR
A. qPCR is performed in a closed-tube system, avoiding a false positive due to PCR product contamination.
B. Quantitative detection of genes extends the scope of clinical application.
C. Spectroscopy has further improved sensitivity.
D. Fluorescent probe hybridization has further improved the specificity.
E. The integration of amplification and automatic assay makes the detection more convenient and quick.
The significance of qPCR detection
A. diagnosis at an early stage.
B. monitoring the therapeutic effect of drug.
C. tracking the cause of illness.
D. the epidemiological investigation.
E. serological typing can classify the individual risk potentially and determine treatment options.
The application of qPCR in clinic.
A. the diagnosis of infectious disease.
B. control and observation of mother-to-child transmission.
C. the diagnosis of genetic disease.
D. the diagnosis of tumor.
E. identification of forensic medicine.
The principle of reverse dot blot hybridization (RDB) gene detection.
This technology uses DNA amplification and membrane hybridization technique. The probe is immobilized onto nitrocellulose membrane or nylon membrane respectively. Each probe is blotted and numbered. The primer is labeled with a biotin at the 5’ end in advance, so the DNA samples are labeled with biotin by specific DNA amplification. The probe hybrid with the DNA samples to test and show hybridization signal after corresponding reaction.
The characteristics of reverse dot blot hybridization technique.
A. can analyze multiple sites at the same time.
(1) A number of different probes are spotted in one membrane at the same time.
(2) RDB technique can detect a variety of different genotypes simultaneously.
(3) RDB technique can simultaneously detect multiple mutations in a gene.
B. high sensitivity and specificity.
(1) PCR amplification of target genes improve the sensitivity.
(2) Many specific probes increase specificity.