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The Steps and Principles of Using the PCR Machine

What is a PCR System?

PCR systems are designed to meet the demands of today's molecular biology laboratory. High-quality optics, reliable temperature control systems and strict production processes ensure instrument reliability, precision and accuracy. The main functions of the PCR system include multi-channel PCR detection, melting curve and data analysis and management. All instrument setup and analysis functions are controlled from an intuitive, easy-to-use software interface for quick experiment setup and data analysis. After each thermal cycle, the fluorescence intensity and cycle number display for all samples is continuously updated. After the experiment, the report can be exported and analyzed using Excel software.

 

The Principle of PCR System

During PCR amplification, a specific fluorescent probe is added at the same time as a pair of primers, and the two ends of the probe are respectively labeled with a reporter fluorescent group and a quenching fluorescent group. At the beginning, the probe is completely bound to any single strand of target DNA, the fluorescent signal emitted by the reporter group is absorbed by the quencher group when they are bound on the sequence, and the fluorescent signal cannot be detected. During PCR amplification, the Taq polymerase enzymes possess an exonuclease activity, and as a result, during replication of one of the DNA strands the enzyme will encounter the bound probes and cleave them apart, resulting in the release of the reporter group into solution and be free of absorbing by the quencher group. That is to say, every time a DNA strand is amplified, a fluorescent reporter signal is formed, realizing the complete synchronization of the accumulation of fluorescent signals and the formation of PCR products. After about 40-45 cycles, analyze the final results of signal formation collected in each cycle, and judge the sample results according to the instructions of the PCR reagents.

  

Advantages of DaAn Gene’s PCR System


>> Learn More on DaAn Gene’s PCR System

 

Reference:

Kadri, K. . Polymerase Chain Reaction (PCR): Principle and Applications. In: Nagpal, M. L. , Boldura, O. , Baltă, C. , Enany, S. , editors. Synthetic Biology - New Interdisciplinary Science [Internet]. London: IntechOpen; 2019 [cited 2022 Aug 29]. Available from: https://www.intechopen.com/chapters/67558 doi: 10.5772/intechopen.86491


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