Monkeypox is a smallpox-like, sporadic acute infectious disease caused by the monkeypox virus. Monkeypox virus is an animal-derived virus that belongs to the Orthopoxviridae family with the variola virus. At present, due to the mutation of the virus, it is subdivided into the Monkeypox Virus Clade One (I) and the Monkeypox Virus Clade Two (II). The Monkeypox Virus Clade One (I) is more virulent, with a fatality rate of 10%, while the Monkeypox Virus Clade Two (II) has a lethality rate of 1%.
Monkeypox is resistant to drying and low temperatures and can survive for several months on soil, crusts, and clothing. The virus is heat sensitive and can be inactivated by heating to 56°C for 30 minutes or 60°C for 10 minutes. It can be inactivated by ultraviolet rays and general disinfectants, and it is sensitive to sodium hypochlorite, chloroxylenol, glutaraldehyde, formaldehyde, and paraformaldehyde.
Since May 2022, the monkeypox virus epidemic has occurred in many countries such as the UK, Portugal, Spain, Australia, Germany and France, which arouse a great deal of public attention. On July 23, the World Health Organization (who) announced that the monkeypox epidemic was listed as a "Public Health Emergency of International Concern (PHEIC)", with the same level as polio and covid-19.
Until September 5, 2022, the global confirmed cases of monkeypox have reached 53027, and it seems the reported cases tend to grow continuously.
The precise diagnosis is an important step to control the outbreak of monkeypox virus, WHO recommended using nucleic acid amplification testing (NAAT) method to detect the monkeypox virus, real-time or conventional polymerase chain reaction (PCR) is the gold standard for monkeypox virus DNA detection. DaAn Gene’s Detection Kit for Monkeypox Virus DNA is based on the WHO recommended diagnosis method, real-time polymerase chain reaction (PCR), performing high specificity and sensitivity.