Hepatitis B is viral hepatitis, which is one of the most common critical liver infections in the world. Hepatitis B is caused by the hepatitis B virus (HBV), and the HBV attacks the liver triggering inflammation that can set off liver damage that can cause cirrhosis, liver cancer, and even death.
Structure of the Hepatitis B Virus
The hepatitis B virus contains an external shell and an internal nucleus.
The external envelope of the virus consists of a surface protein called hepatitis B surface antigen or "HBsAg". The HBsAg can be detected through a blood test , HBsAg-positive test result indicates someone is infected with HBV.
The internal nucleus of HBV is a protein shell known as the hepatitis B core antigen or "HBcAg," which contains the HBV DNA and enzymes used in the replication of the viral.
Hepatitis B infection can be divided into acute hepatitis B and chronic hepatitis B.
Acute Hepatitis B
Acute hepatitis B is a short-term disease condition that occurs in the first six months of exposure to HBV. Certain individuals with acute hepatitis B may be asymptomatic or mildly ill. In some cases, acute hepatitis B may cause liver failure and a more serious illness that requires hospitalization.
Chronic Hepatitis B
Chronic hepatitis B (Chronic means long-lasting over six months.), which leads to inflammation, cirrhosis, and liver cancer and is possibly fatal.
Accurate diagnosis is vital to control hepatitis B infection. Quantification Hepatitis B virus (HBV) DNA plays a critical role in the management of HBV infections. PCR-based assays are the most widely used method for HBV DNA quantification. A sensitive and accurate HBV DNA quantification assay is essential for monitoring hepatitis B virus (HBV) replication.
Here are the Steps for HBV DNA Quantitative Detection:
Blood sampling for detection of hepatitis B virus DNA
Detecting the hepatitis B virus DNA
Observe the viral load. If the viral load is high, it indicates the HBV is actively replicated in the patient's body.
DaAn Gene is the first Chinese company to obtain the CE List A approval for the hepatitis B and C test kits As the world-leading hepatitis PCR detection kit provider, DaAn Gene utilizes its expertise to promote hepatitis care. Accurate diagnosis is important in hepatitis treatment, DaAn Gene dedicates to providing reliable and efficient solutions for viral hepatitis diagnosis. As the world-leading hepatitis PCR detection kit provider, DaAn Gene utilizes its expertise to promote hepatitis care. Accurate diagnosis is important in hepatitis treatment, DaAn Gene dedicates to providing reliable and efficient solutions for viral hepatitis diagnosis.
Feature of DaAn Gene Viral Hepatitis Detection Kits:
Highly sensitive LoD: 10 IU/mL (HBV detection kit) and 20 IU/mL (HCV detection kit).
Quality control: Internal control to monitor the whole NAT procedure.
Shorter window period: Shorter window period (diagnostic period) owing to the adoption of PCR-based technology.
Wide linear range: Wide linear range from 20 to more than 1.0×109 IU/mL (HBV detection kit) and from 50 to more than 1.0×108 IU/mL (HCV detection kit).
Fully automatic nucleic acid extraction: Compatible with automated extraction platform for total nucleic acid purification.
No cross-reactivity: Accuracy can be guaranteed as the kits are free of cross-reactivity with other viruses.
Liang TJ. Hepatitis B: the virus and disease. Hepatology. 2009 May;49(5 Suppl):S13-21. doi: 10.1002/hep.22881. PMID: 19399811; PMCID: PMC2809016.